Product Info Summary
SKU: | A14435-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ABHD4 Antibody Picoband™
SKU/Catalog Number
A14435-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABHD4 Antibody Picoband™ catalog # A14435-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ABHD4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14435-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ABHD4 recombinant protein (Position: Q27-D342).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A14435-1 is reactive to ABHD4 in Human, Mouse, Rat
Applications
A14435-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.794kDa
Background of ABHD4
Predicted to enable lysophosphatidic acid acyltransferase activity and lysophospholipase activity. Predicted to be involved in N-acylphosphatidylethanolamine metabolic process; lipid homeostasis; and phosphatidic acid biosynthetic process. Predicted to act upstream of or within N-acylethanolamine metabolic process. Predicted to be located in endoplasmic reticulum membrane. Predicted to be active in lipid droplet and mitochondrion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABHD4 using anti-ABHD4 antibody (A14435-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABHD4 antigen affinity purified polyclonal antibody (Catalog # A14435-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABHD4 at approximately 39 kDa. The expected band size for ABHD4 is at 39 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of HEL cells using anti-ABHD4 antibody (A14435-1).
Overlay histogram showing HEL cells stained with A14435-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABHD4 Antibody (A14435-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ABHD4 (Source: Uniprot.org, NCBI)
Gene Name
ABHD4
Full Name
(Lyso)-N-acylphosphatidylethanolamine lipase
Weight
38.794kDa
Superfamily
peptidase S33 family
Alternative Names
ABH4; abhydrolase domain containing 4; abhydrolase domain-containing protein 4; Alpha/beta-hydrolase 4; EC 3.1.1.-; FLJ12816; Lyso-N-acylphosphatidylethanolamine lipase ABHD4 ABH4 abhydrolase domain containing 4, N-acyl phospholipase B (Lyso)-N-acylphosphatidylethanolamine lipase|abhydrolase domain-containing protein 4|alpha/beta hydrolase domain-containing protein 4|alpha/beta-hydrolase 4|lyso-N-acylphosphatidylethanolamine lipase|protein ABHD4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABHD4, check out the ABHD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABHD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABHD4 Antibody Picoband™ (A14435-1)
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